Correia J.J., Detrich H.W., III (Еds.) Biophysical Tools for Biologists, Vol. 1 - In Vitro Techniques

Academic Press, Elsevier, 2008. — 996 p. — ISBN: 978-0-12-372520-2
Methods in Cell Biology, Vol.84Dedication
Solution Methods
Binding: A Polemic and Rough Guide
Linked Equilibria in Regulation of Transcription Initiation
Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions
Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions
DiVerential Scanning Calorimetry
Analytical Ultracentrifugation: Sedimentation Velocity and Sedimentation Equilibrium
Determination of Membrane Protein Molecular Weights and Association Equilibrium Constants Using Sedimentation Equilibrium and Sedimentation Velocity
Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods
Applications of Fluorescence Anisotropy to the Study of Protein–DNA Interactions
Circular Dichroism and Its Application to the Study of Biomolecules
Protein Folding and Stability Using Denaturants
Hydrodynamic Modeling: The Solution Conformation of Macromolecules and Their Complexes
X-Ray and Neutron Scattering Data and Their Constrained Molecular Modeling
Structural Investigations into Microtubule-MAP Complexes
Rapid Kinetic Techniques
Mutagenic Analysis of Membrane Protein Functional Mechanisms:
Quantifying DNA–Protein Interactions by Single Molecule Stretching
Isotopomer-Based Metabolomic Analysis by NMR and Mass Spectrometry
Following Molecular Transitions with Single Residue Spatial and Millisecond Time Resolution
Methods and Applications of Site-Directed Spin Labeling EPR Spectroscopy
Fluorescence Correlation Spectroscopy and Its Application to the Characterization of Molecular Properties and Interactions
A Practical Guide on How Osmolytes Modulate Macromolecular Properties
Computational Methods
Stupid Statistics!
Nonlinear Least-Squares Fitting Methods
Methods for Simulating the Dynamics of Complex Biological Processes
Computational Methods for Biomolecular Electrostatics
Ligand EVects on the Protein Ensemble: Unifying the Descriptions of Ligand Binding,
Molecular Modeling of the Cytoskeleton
Mathematical Modeling of Cell Migration